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addgene aav1 cag tdtomato  (Addgene inc)


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    Structured Review

    Addgene inc addgene aav1 cag tdtomato
    Addgene Aav1 Cag Tdtomato, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 279 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/codon/pAAV-CAG-tdTomato+(codon+diversified)+(Plasmid+%2359462)/10__1523_slash_eneuro__0227___26__2026-50-10-10
    Average 96 stars, based on 279 article reviews
    addgene aav1 cag tdtomato - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: N121T and N121S substitutions on the SARS-CoV-2 spike protein impact on serum neutralization.
    Article Snippet: Funding information Ministry of Science and ICT, South Korea, Grant/Award Number: 2020R1A6A1A06046235; Ministry of Education, republic of korea; National Research Foundation of Korea Abstract The N121 site on the spike protein of SARS‐CoV‐2 is associated with heme and its metabolite, biliverdin, which can affect antibody binding.. Both N121T and N121S substitutions have been observed in natural conditions and in a hamster model of dual infection with SARS‐CoV‐2 and Influenza A virus.. Serum pseudotype neutralization assays against HIV‐1 particles carrying wild‐type, N121T, and N121S spikes with immune mouse and human sera revealed that N121T and N121S mutations had a greater impact on serum neutralization than biliverdin treatment.

    Article Title: Multistep allelic conversion in mouse pre-implantation embryos by AAV vectors
    Article Snippet: .. To clone pAAV-CMV version of recombinase plasmids using In-Fusion cloning (Takara Bio) method, codon-optimized Cre sequence was ampli ed from pCAG-Cre-IRES2-GFP (Addgene plasmid #26646) with AAV CDS Cre fw and rv primers, and cloned into pAAV-GFP Control Vector (AAV-400, Cellbiolabs Inc, San Diego, CA) using EcoRI and XbaI restriction sites. ..

    Construct:

    Article Title: N121T and N121S substitutions on the SARS-CoV-2 spike protein impact on serum neutralization.
    Article Snippet: Funding information Ministry of Science and ICT, South Korea, Grant/Award Number: 2020R1A6A1A06046235; Ministry of Education, republic of korea; National Research Foundation of Korea Abstract The N121 site on the spike protein of SARS‐CoV‐2 is associated with heme and its metabolite, biliverdin, which can affect antibody binding.. Both N121T and N121S substitutions have been observed in natural conditions and in a hamster model of dual infection with SARS‐CoV‐2 and Influenza A virus.. Serum pseudotype neutralization assays against HIV‐1 particles carrying wild‐type, N121T, and N121S spikes with immune mouse and human sera revealed that N121T and N121S mutations had a greater impact on serum neutralization than biliverdin treatment.

    Luciferase:

    Article Title: N121T and N121S substitutions on the SARS-CoV-2 spike protein impact on serum neutralization.
    Article Snippet: Funding information Ministry of Science and ICT, South Korea, Grant/Award Number: 2020R1A6A1A06046235; Ministry of Education, republic of korea; National Research Foundation of Korea Abstract The N121 site on the spike protein of SARS‐CoV‐2 is associated with heme and its metabolite, biliverdin, which can affect antibody binding.. Both N121T and N121S substitutions have been observed in natural conditions and in a hamster model of dual infection with SARS‐CoV‐2 and Influenza A virus.. Serum pseudotype neutralization assays against HIV‐1 particles carrying wild‐type, N121T, and N121S spikes with immune mouse and human sera revealed that N121T and N121S mutations had a greater impact on serum neutralization than biliverdin treatment.

    Synthesized:

    Article Title: N121T and N121S substitutions on the SARS-CoV-2 spike protein impact on serum neutralization.
    Article Snippet: Funding information Ministry of Science and ICT, South Korea, Grant/Award Number: 2020R1A6A1A06046235; Ministry of Education, republic of korea; National Research Foundation of Korea Abstract The N121 site on the spike protein of SARS‐CoV‐2 is associated with heme and its metabolite, biliverdin, which can affect antibody binding.. Both N121T and N121S substitutions have been observed in natural conditions and in a hamster model of dual infection with SARS‐CoV‐2 and Influenza A virus.. Serum pseudotype neutralization assays against HIV‐1 particles carrying wild‐type, N121T, and N121S spikes with immune mouse and human sera revealed that N121T and N121S mutations had a greater impact on serum neutralization than biliverdin treatment.

    Expressing:

    Article Title: N121T and N121S substitutions on the SARS-CoV-2 spike protein impact on serum neutralization.
    Article Snippet: Funding information Ministry of Science and ICT, South Korea, Grant/Award Number: 2020R1A6A1A06046235; Ministry of Education, republic of korea; National Research Foundation of Korea Abstract The N121 site on the spike protein of SARS‐CoV‐2 is associated with heme and its metabolite, biliverdin, which can affect antibody binding.. Both N121T and N121S substitutions have been observed in natural conditions and in a hamster model of dual infection with SARS‐CoV‐2 and Influenza A virus.. Serum pseudotype neutralization assays against HIV‐1 particles carrying wild‐type, N121T, and N121S spikes with immune mouse and human sera revealed that N121T and N121S mutations had a greater impact on serum neutralization than biliverdin treatment.

    other:

    Article Title: Identification and structural characterization of small-molecule inhibitors of PINK1
    Article Snippet: The TcPINK1 plasmids used spans residues 121-570 or 128-570 and are codon optimized for expression in E.coli ( ).TcPINK1 (121-570) ampicillin-resistant plasmids were co-expressed in BL21 DE3 E. coli with lambda phosphatase spectinomycin-resistant plasmid (Addgene plasmid #79748).

    Article Title: Estrogen Receptor α/14-3-3 molecular glues as alternative treatment strategy for endocrine resistant breast cancer
    Article Snippet: T47D, ZR-75-01, MDA-MB-231 and HEK293T cells were purchased from ATCC.

    Derivative Assay:

    Article Title: Truncated FGFR2 is a clinically actionable oncogene in multiple cancers
    Article Snippet: Data were analysed using FlowJo (v.10.7.1, BD Biosciences). .. The SIN.LV.SF, SIN.LV.SF-T2A-Puro, SIN.LV.SF-GFP-T2A-Puro lentiviral vectors and the SIN.LV.SF-Cre (Lenti-Cre), SIN.LV.SF-P2A-Cre and pGIN sgPten–P2A-Cre lentivectors all encoding improved cre with mammalian codon usage (derived from pBOB-CAG-iCRE-SD, 12336, Addgene) and the last-mentioned encoding a validated sgRNA targeting E7 of Pten (sgPten) were all previously described , , , . .. For IHC staining, antigen retrieval was performed with citrate buffer (CBB999, ScyTek) at pH 6 (FGF3, FGFR2, PTEN) or Tris-EDTA at pH 9 (MYC, Cyclin D1, E-cadherin, P53).

    Article Title: A kinase translocation reporter reveals real-time dynamics of ERK activity in Drosophila
    Article Snippet: .. ERK-KTR sequences derived from the sequence published by (Addgene #59138) were codon-optimised for Drosophila and synthesised by Thermo Fisher Scientific GeneArt and flanked by EcoRI and SalI sites. ..

    Cloning:

    Article Title: Multistep allelic conversion in mouse pre-implantation embryos by AAV vectors
    Article Snippet: .. To clone pAAV-CMV version of recombinase plasmids using In-Fusion cloning (Takara Bio) method, codon-optimized Cre sequence was ampli ed from pCAG-Cre-IRES2-GFP (Addgene plasmid #26646) with AAV CDS Cre fw and rv primers, and cloned into pAAV-GFP Control Vector (AAV-400, Cellbiolabs Inc, San Diego, CA) using EcoRI and XbaI restriction sites. ..

    Sequencing:

    Article Title: Multistep allelic conversion in mouse pre-implantation embryos by AAV vectors
    Article Snippet: .. To clone pAAV-CMV version of recombinase plasmids using In-Fusion cloning (Takara Bio) method, codon-optimized Cre sequence was ampli ed from pCAG-Cre-IRES2-GFP (Addgene plasmid #26646) with AAV CDS Cre fw and rv primers, and cloned into pAAV-GFP Control Vector (AAV-400, Cellbiolabs Inc, San Diego, CA) using EcoRI and XbaI restriction sites. ..

    Article Title: A kinase translocation reporter reveals real-time dynamics of ERK activity in Drosophila
    Article Snippet: .. ERK-KTR sequences derived from the sequence published by (Addgene #59138) were codon-optimised for Drosophila and synthesised by Thermo Fisher Scientific GeneArt and flanked by EcoRI and SalI sites. ..

    Bioprocessing:

    Article Title: Multistep allelic conversion in mouse pre-implantation embryos by AAV vectors
    Article Snippet: .. To clone pAAV-CMV version of recombinase plasmids using In-Fusion cloning (Takara Bio) method, codon-optimized Cre sequence was ampli ed from pCAG-Cre-IRES2-GFP (Addgene plasmid #26646) with AAV CDS Cre fw and rv primers, and cloned into pAAV-GFP Control Vector (AAV-400, Cellbiolabs Inc, San Diego, CA) using EcoRI and XbaI restriction sites. ..

    Clone Assay:

    Article Title: Multistep allelic conversion in mouse pre-implantation embryos by AAV vectors
    Article Snippet: .. To clone pAAV-CMV version of recombinase plasmids using In-Fusion cloning (Takara Bio) method, codon-optimized Cre sequence was ampli ed from pCAG-Cre-IRES2-GFP (Addgene plasmid #26646) with AAV CDS Cre fw and rv primers, and cloned into pAAV-GFP Control Vector (AAV-400, Cellbiolabs Inc, San Diego, CA) using EcoRI and XbaI restriction sites. ..

    Control:

    Article Title: Multistep allelic conversion in mouse pre-implantation embryos by AAV vectors
    Article Snippet: .. To clone pAAV-CMV version of recombinase plasmids using In-Fusion cloning (Takara Bio) method, codon-optimized Cre sequence was ampli ed from pCAG-Cre-IRES2-GFP (Addgene plasmid #26646) with AAV CDS Cre fw and rv primers, and cloned into pAAV-GFP Control Vector (AAV-400, Cellbiolabs Inc, San Diego, CA) using EcoRI and XbaI restriction sites. ..



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